Flow Induced Dispersion Analysis (FIDA) is a new proprietary technology developed for quantification and characterization of proteins (including biologics), early detection of immune responses (including immunogenicity testing) and affinity assessment. FIDA is characterized by being fast (minutes), requiring very small sample amounts (nL-mL), and being exceptionally tolerant to the sample matrix. Contrary to most other procedures, the FIDA methodology is based on binding in homogenous solution; complications related to non-specific surface adsorption and challenging assay development is therefore avoided. The unique features of FIDA enable characterization and quantification in native (biorelevant) environments and in-built assay quality control and automation.
FIDA is based on dispersion analysis of a parabolic hydrodynamic flow profile. The dispersion of an injected sample zone of an indicator depends on flow channel dimensions, flow rate and apparent diffusivity of the indicator. Dedicated FIDA instrumentation and software allows accurate quantification of the apparent diffusivity of the indicator in different environments. Particularly, the interaction with an analyte such as an antibody based drug may be quantified. The principle is illustrated in the figure below.
Nicklas N. Poulsen, Nina Z. Andersen, Jesper Østergaard, Guisheng Zhuang, Nickolaj J. Petersen and Henrik Jensen: Flow Induced Dispersion Analysis Rapidly Quantifies Proteins in Human Plasma Samples, Analyst, 2015, 140, 4365-4369.
Henrik Jensen, Susan W. Larsen, Claus S. Larsen and Jesper Østergaard: Physicochemical profiling of drug candidates using capillary-based techniques, Journal of Drug Delivery Science and Technology, 2013, 23, 333-345.
Henrik Jensen and Jesper Østergaard: Flow Induced Dispersion Analysis Quantifies Noncovalent Interactions in Nanoliter Samples, J. Am. Chem. Soc., 2010, 132, 4070-4071